Research, Scholarship, Creative Work in Progress

Studies of Structure-Function Relationships in Transcriptional Pausing by Escherichia coli RNA Polymerase - Julia Nguyen '15 and Laura Ojeda '15

Use of Nucleotide Analogs to Analyze the Effect of a Downstream A-tract on Transcriptional Pausing by E. coli RNA Polymerase In Vitro - Amanda Lee '12

Use of Sequence Swapping to Dissect the Effect of Downstream Sequences on Transcriptional
Pausing by E. coli RNA Polymerase In Vitro - Reema Khanchandani '11

Role of the Non-template Strand in the Effect of a Downstream A-tract on Transcriptional Pausing by E. coli RNA Polymerase In Vitro - Chandana Lanka '10

Use of Site-Directed Mutagenesis to Construct a Mutant E. coli RNA Polymerase Altered in a Region that Contacts Downstream DNA - Laura Marino '09

Publications

Lanka, C. (Goucher ’10), Marino, L. (Goucher ’09), Palangat, M., Landick, R. and Levin, J., “The Effect of A-Tract-Induced DNA Bending on Transcriptional Elongation by E. coli RNA Polymerase,” Poster Presentation, Federation of American Societies for Experimental Biology Conference on “Mechanisms and Regulation of Prokaryotic Transcription,” Saxtons River, VT, June 21-26, 2009.

Wang, Q., Tullius, T.D. and Levin, J.R., “Effects of Discontinuities in the DNA Template on Abortive Initiation and Promoter Escape by Escherichia coli RNA Polymerase,” Journal of Biological Chemistry, 282, 26917-26927, 2007.

Levin, J.R., Blake, J.J., Ganunis, R.A. and Tullius, T.D., “The Roles of Specific Template Nucleosides in the Formation of Stable Transcription Complexes by Escherichia coli RNA Polymerase,” Journal of Biological Chemistry, 275, 6885-6893, 2000.

External Awards, Honors, Grants

NIH R15 (AREA) Research Grant, $150,000, "The Effect of DNA Bending on Transcriptional Elongation by E. coli RNA Polymerase," 2007-2010

NIH American Recovery & Reinvestment Act Administrative Supplement, $69,439, "The Effect of DNA Bending on Transcriptional Elongation by E. coli RNA Polymerase," 2009-2010

Research Corporation Cottrell College Science Award, $34,000, "Chemical footprinting studies of transcriptional elongation by E. coli RNA polymerase," 1992-1994

Invited Talks

Studies of Structure-Function Relationships in Transcriptional Pausing by Escherichia coli RNA Polymerase, 16th Annual UMBC Undergraduate Research Symposium in the Chemical and Biological Sciences, Catonsville, MD (with *J Nguyen and *L Ojeda) 2013

Investigation of Downstream DNA Effects on Elongational Pausing by E. coli RNA Polymerase, 12th Annual UMBC Undergraduate Research Symposium in the Chemical and Biological Sciences, Catonsville, MD (with *C Lanka, *R Khanchandani, and *C. Crook) 2009

The Effect of A-Tract-Induced DNA Bending on Transcriptional Elongation by E. coli RNA Polymerase, Federation of American Societies for Experimental Biology (FASEB) Conference on "Mechanisms and Regulation of Prokaryotic Transcription," Saxtons River, VT, June 21-26, 2009 (with *C Lanka, *L Marino, M Palangat and R Landick)

The Effect of A-Tract-Induced DNA Bending on Transcriptional Elongation by E. coli RNA Polymerase, 11th Annual UMBC Undergraduate Research Symposium in the Chemical and Biological Sciences, Catonsville, MD (with *C Lanka and *L Marino) 2008

Effects of A-Tract-Directed DNA Bending on Transcriptional Elongation by Escherichia coli RNA Polymerase, FASEB Conference on "Mechanisms & Regulation of Prokaryotic Transcription," Saxtons River, VT, June 23-28, 2007 (poster, with *V Tucker)

Effect of Single Nucleoside Template Gaps on Abortive Initiation and Promoter Escape by E. coli RNA Polymerase, FASEB Conference on "Mechanisms & Regulation of Prokaryotic Transcription," Saxtons River, VT, June 18-23, 2005 (poster, with Q Wang and T Tullius)

* undergraduate student co-authors